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SEC24A Recombinant antibody, PBS Only

SEC24A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230105B3

Cat no : 82908-1-PBS

Print datasheet

Synonyms

SEC24-related protein A, Protein transport protein Sec24A, 230105B3

验证数据展示

  • WB analysis using 82908-1-RR (same clone as 82908-1-PBS)

    WB analysis using 82908-1-RR (same clone as 82908-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82908-1-RR (SEC24A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82908-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82908-1-RR (same clone as 82908-1-PBS)

    IF Staining of HeLa using 82908-1-RR (same clone as 82908-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC24A antibody (82908-1-RR, Clone: 230105B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82908-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82908-1-RR (same clone as 82908-1-PBS)

    FC experiment of HepG2 using 82908-1-RR (same clone as 82908-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SEC24A (82908-1-RR, Clone:230105B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82908-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82908-1-RR

    Affinity and Kinetic Characterization of 82908-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82908-1-RR against Human SEC24A were performed. The affinity constant is below 1 pM.

产品信息

82908-1-PBS targets SEC24A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen SEC24A fusion protein Ag8723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SEC24 family, member A (S. cerevisiae)
Synonyms SEC24-related protein A, Protein transport protein Sec24A, 230105B3
Calculated Molecular Weight 613 aa, 66 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC019341
Gene Symbol SEC24A
Gene ID (NCBI) 10802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95486
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.