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PARK7/DJ-1 Recombinant antibody

PARK7/DJ-1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230124E12

Cat no : 82913-1-RR

Print datasheet

Synonyms

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

验证数据展示

  • WB analysis using 82913-1-RR

    WB analysis using 82913-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82913-1-RR (PARK7/DJ-1 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82913-1-RR

    IP experiment of HeLa using 82913-1-RR

    IP result of anti-PARK7/DJ-1 (IP:82913-1-RR, 4ug; Detection:82913-1-RR 1:2000) with HeLa cells lysate 1920 ug.

  • IF Staining of HepG2 using 82913-1-RR

    IF Staining of HepG2 using 82913-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PARK7/DJ-1 antibody (82913-1-RR, Clone: 230124E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 82913-1-RR

    FC experiment of HepG2 using 82913-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 82913-1-RR

    FC experiment of HeLa using 82913-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82913-1-RR

    Affinity and Kinetic Characterization of 82913-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82913-1-RR against Human PARK7/DJ-1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PARK7 Ready-To-Use IHC Kit. PARK7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82913-1-RR targets PARK7/DJ-1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen PARK7/DJ-1 fusion protein Ag2287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Parkinson disease (autosomal recessive, early onset) 7
Synonyms PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1
Calculated Molecular Weight 189 aa, 20 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC008188
Gene Symbol PARK7
Gene ID (NCBI) 11315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

实验方案

Product Specific Protocols
WB protocol for PARK7/DJ-1 antibody 82913-1-RRDownload protocol
IF protocol for PARK7/DJ-1 antibody 82913-1-RRDownload protocol
IP protocol for PARK7/DJ-1 antibody 82913-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols