IHCeasy PARK7 Ready-To-Use IHC Kit

PARK7 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC0557

Synonyms

DJ 1, DJ1, DJ-1, Oncogene DJ1, PARK7, PARK7,DJ-1, Parkinson disease protein 7, Protein DJ 1



Product Information

KHC0557 is a ready-to-use IHC kit for staining of PARK7. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy PARK7 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms DJ 1, DJ1, DJ-1, Oncogene DJ1, PARK7, PARK7,DJ-1, Parkinson disease protein 7, Protein DJ 1