Alpha-1-Antitrypsin Recombinant antibody
Alpha-1-Antitrypsin Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
230109A8
验证数据展示
经过测试的应用
Positive WB detected in | human liver tissue, human heart tissue, human saliva, human placenta tissue, human urine |
Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82918-1-RR targets Alpha-1-Antitrypsin in WB, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | Alpha-1-Antitrypsin fusion protein Ag9369 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
Synonyms | A1A, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT |
Calculated Molecular Weight | 418 aa, 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC015642 |
Gene Symbol | Alpha 1-Antitrypsin |
Gene ID (NCBI) | 5265 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P01009 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Alpha-1-Antitrypsin antibody 82918-1-RR | Download protocol |
FC protocol for Alpha-1-Antitrypsin antibody 82918-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |