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验证数据展示

  • WB analysis using 82918-1-RR (same clone as 82918-1-PBS)

    WB analysis using 82918-1-RR (same clone as 82918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82918-1-RR (same clone as 82918-1-PBS)

    WB analysis of HepG2 using 82918-1-RR (same clone as 82918-1-PBS)

    WB result of Alpha-1-Antitrypsin antibody (82918-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha-1-Antitrypsin transfected HepG2 cells. This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82918-1-RR (same clone as 82918-1-PBS)

    IHC staining of human colon using 82918-1-RR (same clone as 82918-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of human stomach using 82918-1-RR (same clone as 82918-1-PBS)

    IHC staining of human stomach using 82918-1-RR (same clone as 82918-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • FC experiment of HepG2 using 82918-1-RR (same clone as 82918-1-PBS)

    FC experiment of HepG2 using 82918-1-RR (same clone as 82918-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-1-Antitrypsin Recombinant antibody (82918-1-RR, Clone:230109A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82918-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82918-1-RR

    Affinity and Kinetic Characterization of 82918-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82918-1-RR against Human Alpha-1-Antitrypsin were performed. The affinity constant is 0.719 nM.

  • Featured Product
  • KD/KO验证

Alpha-1-Antitrypsin Recombinant antibody, PBS Only

Alpha-1-Antitrypsin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82918-1-PBS

产品说明书

CloneNo. 230109A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.

产品信息

82918-1-PBS targets Alpha-1-Antitrypsin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Alpha-1-Antitrypsin fusion protein Ag9369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
别名 a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT
计算分子量 418 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC015642
基因名称 Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01009
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.