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  • Featured Product
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Alpha-1-Antitrypsin Recombinant antibody

Alpha-1-Antitrypsin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230109A8

Cat no : 82918-1-RR

Print datasheet

Synonyms

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

验证数据展示

  • WB analysis using 82918-1-RR

    WB analysis using 82918-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82918-1-RR

    WB analysis of HepG2 using 82918-1-RR

    WB result of Alpha-1-Antitrypsin antibody (82918-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha-1-Antitrypsin transfected HepG2 cells.

  • IHC staining of human colon using 82918-1-RR

    IHC staining of human colon using 82918-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • IHC staining of human stomach using 82918-1-RR

    IHC staining of human stomach using 82918-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82918-1-RR (Alpha-1-Antitrypsin antibody) at dilution of 1:6400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINA1/AAT antibody included
  • FC experiment of HepG2 using 82918-1-RR

    FC experiment of HepG2 using 82918-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-1-Antitrypsin Recombinant antibody (82918-1-RR, Clone:230109A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82918-1-RR

    Affinity and Kinetic Characterization of 82918-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82918-1-RR against Human Alpha-1-Antitrypsin were performed. The affinity constant is 0.719 nM.

经过测试的应用

Positive WB detected inhuman liver tissue, HepG2 cells, human heart tissue, human saliva, human placenta tissue, human urine
Positive IHC detected inhuman colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3200-1:12800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82918-1-RR targets Alpha-1-Antitrypsin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Alpha-1-Antitrypsin fusion protein Ag9369 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Synonyms a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT
Calculated Molecular Weight 418 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC015642
Gene Symbol Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01009
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

实验方案

Product Specific Protocols
WB protocol for Alpha-1-Antitrypsin antibody 82918-1-RRDownload protocol
IHC protocol for Alpha-1-Antitrypsin antibody 82918-1-RRDownload protocol
FC protocol for Alpha-1-Antitrypsin antibody 82918-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols