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GSDMA Recombinant antibody

GSDMA Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230161D4

Cat no : 82920-1-RR

Print datasheet

Synonyms

Gasdermin-A, Gasdermin-1, gasdermin A, Gasdermin 1, FKSG9

验证数据展示

  • WB analysis of Daudi using 82920-1-RR

    WB analysis of Daudi using 82920-1-RR

    Daudi cells were subjected to SDS PAGE followed by western blot with 82920-1-RR (GSDMA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82920-1-RR

    Affinity and Kinetic Characterization of 82920-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82920-1-RR against Human GSDMA were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inDaudi cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82920-1-RR targets GSDMA in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen GSDMA fusion protein Ag24407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gasdermin A
Synonyms Gasdermin-A, Gasdermin-1, gasdermin A, Gasdermin 1, FKSG9
Calculated Molecular Weight 445 aa, 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC109197
Gene Symbol GSDMA
Gene ID (NCBI) 284110
RRIDAB_3670655
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96QA5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.

实验方案

Product Specific Protocols
WB protocol for GSDMA antibody 82920-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols