验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, BxPC-3 cells, U2OS cells, mouse colon tissue, mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82926-1-RR targets RNF160 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag29467 Product name: Recombinant human RNF160 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC140790 Sequence: MGGKNKQRTKGNLRPSNSGRAAELLAKEQGTVPGFIGFGTSQSDLGYVPAIQGAEEIDSLVDSDFRMVLRKLSKKDVTTKLKAMQEFGTMCTERDTETVKGVLPYWPRIFCKISLDHDRRVREATQQAFEKLILKVKKQLAPYLKSLMGYWLMAQCDTYTPAAFAAKDAFEAAFPPSKQPEAIAFCKDEITSVLQDHLIKETPDTLSDPQTVPEEEREAKFYRVVTCSLLALKRLLCLLPDNELDSLEEKFKSLLSQNKFWKYGKHSVPQIRSAYFELVSALCQRIPQLMKEEASKVSPS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ring finger protein 160 |
| 别名 | 230075H2, C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27 |
| 观测分子量 | 180-200 kDa |
| GenBank蛋白编号 | BC140790 |
| 基因名称 | RNF160 |
| Gene ID (NCBI) | 26046 |
| RRID | AB_3670664 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94822 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RNF160 antibody 82926-1-RR | Download protocol |
| WB protocol for RNF160 antibody 82926-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
