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RNF160 Recombinant antibody, PBS Only

RNF160 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230075H2

Cat no : 82926-1-PBS

Print datasheet

Synonyms

HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10

验证数据展示

  • WB analysis using 82926-1-RR (same clone as 82926-1-PBS)

    WB analysis using 82926-1-RR (same clone as 82926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82926-1-RR (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 82926-1-RR (same clone as 82926-1-PBS)

    IHC staining of mouse colon using 82926-1-RR (same clone as 82926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82926-1-RR (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82926-1-RR (same clone as 82926-1-PBS)

    FC experiment of U2OS using 82926-1-RR (same clone as 82926-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RNF160 Recombinant antibody (82926-1-RR, Clone:230075H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82926-1-RR

    Affinity and Kinetic Characterization of 82926-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82926-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

产品信息

82926-1-PBS targets RNF160 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RNF160 fusion protein Ag29467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 160
Synonyms HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10
Observed Molecular Weight180-200 kDa
GenBank Accession NumberBC140790
Gene Symbol RNF160
Gene ID (NCBI) 26046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94822
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).