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RNF160 Recombinant antibody

RNF160 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230075H2

Cat no : 82926-1-RR

Print datasheet

Synonyms

HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10

验证数据展示

  • WB analysis using 82926-1-RR

    WB analysis using 82926-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82926-1-RR (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 82926-1-RR

    IHC staining of mouse colon using 82926-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82926-1-RR (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U2OS using 82926-1-RR

    FC experiment of U2OS using 82926-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RNF160 Recombinant antibody (82926-1-RR, Clone:230075H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82926-1-RR

    Affinity and Kinetic Characterization of 82926-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82926-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, BxPC-3 cells, U2OS cells, mouse colon tissue, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82926-1-RR targets RNF160 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RNF160 fusion protein Ag29467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 160
Synonyms HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10
Observed Molecular Weight180-200 kDa
GenBank Accession NumberBC140790
Gene Symbol RNF160
Gene ID (NCBI) 26046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94822
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

实验方案

Product Specific Protocols
WB protocol for RNF160 antibody 82926-1-RRDownload protocol
IHC protocol for RNF160 antibody 82926-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols