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RNF160 Recombinant antibody

RNF160 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82926-1-RR

产品说明书

CloneNo. 230075H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, BxPC-3 cells, U2OS cells, mouse colon tissue, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82926-1-RR targets RNF160 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RNF160 fusion protein Ag29467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 160
别名 HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10
观测分子量180-200 kDa
GenBank蛋白编号BC140790
基因名称 RNF160
Gene ID (NCBI) 26046
RRIDAB_3670664
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94822
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

实验方案

Product Specific Protocols
WB protocol for RNF160 antibody 82926-1-RRDownload protocol
IHC protocol for RNF160 antibody 82926-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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