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验证数据展示

  • WB analysis using 82933-1-RR

    WB analysis using 82933-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82933-1-RR (CUX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 82933-1-RR

    WB analysis of SH-SY5Y using 82933-1-RR

    WB result of CUX2 antibody (82933-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUX2 transfected SH-SY5Y cells.

  • IF Staining of HeLa using 82933-1-RR

    IF Staining of HeLa using 82933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 82933-1-RR

    IF Staining of HeLa using 82933-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82933-1-RR

    Affinity and Kinetic Characterization of 82933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82933-1-RR against Human CUX2 were performed. The affinity constant is 1.02 nM.

  • Featured Product
  • KD/KO验证

CUX2 Recombinant antibody

CUX2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82933-1-RR

产品说明书

CloneNo. 230235G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, SH-SY5Y cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82933-1-RR targets CUX2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CUX2 fusion protein Ag18809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cut-like homeobox 2
别名 Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2
计算分子量 1486 aa, 162 kDa
观测分子量 162 kDa
GenBank蛋白编号BC151245
基因名称 CUX2
Gene ID (NCBI) 23316
RRIDAB_3670675
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14529
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cut like homeobox 2 (CUX2) also named as, CDP2 or CUTL2, is a 1486 amino acid protein, which contains one homeobox DNA-binding domain and three CUT DNA-binding domains. CUX2 localizes in the nucleus and belongs to the CUT homeobox family. CUX2 may as a transcription factor be involved in neural specification.

实验方案

Product Specific Protocols
WB protocol for CUX2 antibody 82933-1-RRDownload protocol
IF protocol for CUX2 antibody 82933-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols