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验证数据展示

  • IF Staining of HepG2 using 82937-1-RR

    IF Staining of HepG2 using 82937-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAT5 antibody (82937-1-RR, Clone: 230181D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HCT 116 using 82937-1-RR

    FC experiment of HCT 116 using 82937-1-RR

    1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug Anti-Human KAT5 (82937-1-RR, Clone:230181D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82937-1-RR

    Affinity and Kinetic Characterization of 82937-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82937-1-RR against Human TIP60/KAT5 were performed. The affinity constant is below 1 pM.

TIP60/KAT5 Recombinant antibody

TIP60/KAT5 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82937-1-RR

产品说明书

CloneNo. 230181D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

KAT5, EC:2.3.1.48, EC:2.3.1.-, cPLA2, cPLA(2)-interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy KAT5 Ready-To-Use IHC Kit. KAT5 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82937-1-RR targets TIP60/KAT5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TIP60/KAT5 fusion protein Ag1261 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 K(lysine) acetyltransferase 5
别名 KAT5, EC:2.3.1.48, EC:2.3.1.-, cPLA2, cPLA(2)-interacting protein
计算分子量 60 kDa
GenBank蛋白编号BC000166
基因名称 KAT5
Gene ID (NCBI) 10524
RRIDAB_3670680
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92993
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.

实验方案

Product Specific Protocols
IF protocol for TIP60/KAT5 antibody 82937-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols