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MAX Recombinant antibody

MAX Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230215E3

Cat no : 82958-1-RR

Print datasheet

Synonyms

bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4

验证数据展示

  • WB analysis using 82958-1-RR

    WB analysis using 82958-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-1-RR (MAX antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82958-1-RR

    Affinity and Kinetic Characterization of 82958-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-1-RR against Human MAX were performed. The affinity constant is 0.257 nM.

经过测试的应用

Positive WB detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy MAX Ready-To-Use IHC Kit. MAX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82958-1-RR targets MAX in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MAX fusion protein Ag0680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MYC associated factor X
Synonyms bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4
Calculated Molecular Weight 18 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003525
Gene Symbol MAX
Gene ID (NCBI) 4149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

实验方案

Product Specific Protocols
WB protocol for MAX antibody 82958-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols