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验证数据展示

  • WB analysis using 82964-1-RR

    WB analysis using 82964-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82964-1-RR (URAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 82964-1-RR

    FC experiment of HepG2 using 82964-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug URAT1 Recombinant antibody (82964-1-RR, Clone:230204B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

URAT1 Recombinant antibody

URAT1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82964-1-RR

产品说明书

CloneNo. 230204B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750

规格: 
50 μL
100 μL
150 μL

¥2480/ 100 μL

浓度: 1000 ug/ml

库存：现货

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82964-1-RR targets URAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 URAT1 fusion protein Ag27805 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 22 (organic anion/urate transporter), member 12
别名 SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4
计算分子量 60 kDa
观测分子量 70 kDa
GenBank蛋白编号BC053348
基因名称 URAT1
Gene ID (NCBI) 116085
RRIDAB_3670710
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96S37
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.

实验方案

Product Specific Protocols
WB protocol for URAT1 antibody 82964-1-RRDownload protocol
FC protocol for URAT1 antibody 82964-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols