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验证数据展示

  • WB analysis using CL750-82964

    WB analysis using CL750-82964

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82964 (URAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-82964

    FC experiment of HepG2 using CL750-82964

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated URAT1 Recombinant antibody (CL750-82964, Clone:230204B3)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 750-conjugated URAT1 Recombinant antibody

URAT1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82964

产品说明书

CloneNo. 230204B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, mouse liver tissue
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82964-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82964 targets URAT1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 URAT1 fusion protein Ag27805 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 22 (organic anion/urate transporter), member 12
别名 SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4
计算分子量 60 kDa
观测分子量 70 kDa
GenBank蛋白编号BC053348
基因名称 URAT1
Gene ID (NCBI) 116085
RRIDAB_3673754
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96S37
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 URAT1 antibody CL750-82964Download protocol
FC protocol for CL Plus 750 URAT1 antibody CL750-82964Download protocol
Standard Protocols
Click here to view our Standard Protocols