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验证数据展示

  • WB analysis using CL750-82964

    WB analysis using CL750-82964

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82964 (URAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-82964

    FC experiment of HepG2 using CL750-82964

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated URAT1 Recombinant antibody (CL750-82964, Clone:230204B3)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 750-conjugated URAT1 Recombinant antibody

URAT1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82964

Print datasheet

CloneNo. 230204B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, mouse liver tissue
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82964-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82964 targets URAT1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen URAT1 fusion protein Ag27805 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 22 (organic anion/urate transporter), member 12
Synonyms SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC053348
Gene Symbol URAT1
Gene ID (NCBI) 116085
RRIDAB_3673754
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96S37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 URAT1 antibody CL750-82964Download protocol
FC protocol for CL Plus 750 URAT1 antibody CL750-82964Download protocol
Standard Protocols
Click here to view our Standard Protocols