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验证数据展示

  • FC experiment of U-2 OS using 82966-2-RR

    FC experiment of U-2 OS using 82966-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of HeLa using 82966-2-RR

    FC experiment of HeLa using 82966-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of U2OS using 82966-2-RR

    IF Staining of U2OS using 82966-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ARHGEF19 antibody (82966-2-RR, Clone: 230290F5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Affinity and Kinetic Characterization of 82966-2-RR

    Affinity and Kinetic Characterization of 82966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82966-2-RR against Human ARHGEF19 were performed. The affinity constant is below 1 pM.

ARHGEF19 Recombinant monoclonal antibody

ARHGEF19 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82966-2-RR

产品说明书

CloneNo. 230290F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

230290F5, Ephexin-2, WGEF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82966-2-RR targets ARHGEF19 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11645

Product name: Recombinant human ARHGEF19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 454-802 aa of BC040640

Sequence: VVLDHCPAFRRVYLPYVTNQAYQERTYQRLLLENPRFPGILARLEESPVCQRLPLTSFLILPFQRITRLKMLVENILKRTAQGSEDEDMATKAFNALKELVQECNASVQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKLKLSSKAVYLHLFNDCLLLSRRKELGKFAVFVHAKMAELQVRDLSLKLQGIPGHVFLLQLLHGQHMKHQFLLRARTESEKQRWISALCPSSPQEDKEVISEGEDCPQVQCVRTYKALHPDELTLEKTDILSVRTWTSDGWLEGVRLADGEKGWVPQAYVEEISSLSARLRNLRENKRVTSATSKLGEAPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 19
别名 230290F5, Ephexin-2, WGEF
计算分子量 802 aa, 89 kDa
GenBank蛋白编号BC040640
基因名称 ARHGEF19
Gene ID (NCBI) 128272
RRIDAB_3670712
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IW93
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rho guanine nucleotide exchange factors (RHOGEFs) are proteins that could regulate Rho GTPases, which can be involved in a variety of physiological processes, such as survival, development, differentiation, and motility. ARHGEF19 also known as WGEF, is a member of the RhoGEF family. ARHGEF19 contains two identical functional domains, including the Dbl homology (DH) and pleckstrin homology (PH) domain(PMID:32993957). ARHGEF19 was highly expressed in human breast cancer(PMID: 34813497).

实验方案

Product Specific Protocols
IF protocol for ARHGEF19 antibody 82966-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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