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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of U-2 OS using 82966-2-RR (same clone as 82966-2-PBS)

    FC experiment of U-2 OS using 82966-2-RR (same clone as 82966-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82966-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82966-2-RR (same clone as 82966-2-PBS)

    FC experiment of HeLa using 82966-2-RR (same clone as 82966-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82966-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82966-2-RR (same clone as 82966-2-PBS)

    IF Staining of U2OS using 82966-2-RR (same clone as 82966-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ARHGEF19 antibody (82966-2-RR, Clone: 230290F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82966-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82966-2-RR

    Affinity and Kinetic Characterization of 82966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82966-2-RR against Human ARHGEF19 were performed. The affinity constant is below 1 pM.

ARHGEF19 Recombinant antibody, PBS Only

ARHGEF19 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82966-2-PBS

产品说明书

CloneNo. 230290F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

WGEF, Ephexin-2, 230290F5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82966-2-PBS targets ARHGEF19 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ARHGEF19 fusion protein Ag11645 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 19
别名 WGEF, Ephexin-2, 230290F5
计算分子量 802 aa, 89 kDa
GenBank蛋白编号BC040640
基因名称 ARHGEF19
Gene ID (NCBI) 128272
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IW93
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rho guanine nucleotide exchange factors (RHOGEFs) are proteins that could regulate Rho GTPases, which can be involved in a variety of physiological processes, such as survival, development, differentiation, and motility. ARHGEF19 also known as WGEF, is a member of the RhoGEF family. ARHGEF19 contains two identical functional domains, including the Dbl homology (DH) and pleckstrin homology (PH) domain(PMID:32993957). ARHGEF19 was highly expressed in human breast cancer(PMID: 34813497).