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HMGB1 Recombinant antibody, PBS Only (Capture)

HMGB1 Recombinant Antibody for WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230182G6

Cat No : 82973-1-PBS

Print datasheet

Synonyms

DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

验证数据展示

  • WB analysis using 82973-1-RR (same clone as 82973-1-PBS)

    WB analysis using 82973-1-RR (same clone as 82973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82973-1-RR (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    IF Staining of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGB1 antibody (82973-1-RR, Clone: 230182G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82973-1-RR (same clone as 82973-1-PBS)

    IHC staining of mouse brain using 82973-1-RR (same clone as 82973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HMGB1 antibody included
  • Sandwich ELISA standard curve of MP00311-1

    Sandwich ELISA standard curve of MP00311-1

    Sandwich ELISA standard curve of MP00311-1, HMGB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82973-1-PBS. Detection antibody: 82973-3-PBS. Standard: Ag1264. Range: 0.156-10 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy HMGB1 Ready-To-Use IHC Kit. HMGB1 primary antibody included.

产品信息

82973-1-PBS targets HMGB1 as part of a matched antibody pair:

MP00311-1: 82973-1-PBS capture and 82973-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HMGB1 fusion protein Ag1264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name high-mobility group box 1
Synonyms DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC003378
Gene Symbol HMGB1
Gene ID (NCBI) 3146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09429
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.