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验证数据展示

  • WB analysis using 82993-6-RR

    WB analysis using 82993-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 82993-6-RR

    IHC staining of human ovary cancer using 82993-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82993-6-RR (Endoglin/CD105 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Endoglin/CD105 antibody included

Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82993-6-RR

产品说明书

CloneNo. 241196G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD105, 241196G9, HHT1, ENG, Endoglin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HUVEC cells, JAR cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Endoglin/CD105 Ready-To-Use IHC Kit. Endoglin/CD105 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82993-6-RR targets Endoglin/CD105 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endoglin
别名 CD105, 241196G9, HHT1, ENG, Endoglin
计算分子量 71 kDa
观测分子量75-90 kDa
GenBank蛋白编号BC014271
基因名称 CD105
Gene ID (NCBI) 2022
RRIDAB_3670742
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 82993-6-RRDownload protocol
IHC protocol for Endoglin/CD105 antibody 82993-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols