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ACVR1B Recombinant antibody

ACVR1B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230260E12

Cat no : 83026-5-RR

Print datasheet

Synonyms

Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB

验证数据展示

  • IF Staining of NIH/3T3 using 83026-5-RR

    IF Staining of NIH/3T3 using 83026-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ACVR1B antibody (83026-5-RR, Clone: 230260E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HEK-293 using 83026-5-RR

    FC experiment of HEK-293 using 83026-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ACVR1B (83026-5-RR, Clone:230260E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83026-5-RR

    Affinity and Kinetic Characterization of 83026-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83026-5-RR against Human ACVR1B were performed. The affinity constant is 16.1 nM.

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83026-5-RR targets ACVR1B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen ACVR1B fusion protein Ag0129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activin A receptor, type IB
Synonyms Activin receptor-like kinase 4, Activin receptor type-1B, Activin receptor type 1B, Activin receptor like kinase 4, activin A receptor, type IB
Calculated Molecular Weight 57 kDa
GenBank Accession NumberBC000254
Gene Symbol ACVR1B
Gene ID (NCBI) 91
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).

实验方案

Product Specific Protocols
IF protocol for ACVR1B antibody 83026-5-RRDownload protocol
FC protocol for ACVR1B antibody 83026-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols