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验证数据展示

  • WB analysis of HeLa using 83038-4-RR

    WB analysis of HeLa using 83038-4-RR

    WB result of ABCD1 antibody (83038-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCD1 transfected HeLa cells.

  • WB analysis using 83038-4-RR

    WB analysis using 83038-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83038-4-RR (ABCD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83038-4-RR

    IF Staining of HeLa using 83038-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABCD1 antibody (83038-4-RR, Clone: 230226E12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83038-4-RR

    FC experiment of HeLa using 83038-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ABCD1 Recombinant antibody (83038-4-RR, Clone:230226E12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83038-4-RR

    Affinity and Kinetic Characterization of 83038-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83038-4-RR against Human ABCD1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

ABCD1 Recombinant antibody

ABCD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83038-4-RR

产品说明书

CloneNo. 230226E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, JAR cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCD1 Ready-To-Use IHC Kit. ABCD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83038-4-RR targets ABCD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 ABCD1 fusion protein Ag0453 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 1
别名 EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1
计算分子量 745 aa, 83 kDa
观测分子量 75 kDa
GenBank蛋白编号BC025358
基因名称 ABCD1
Gene ID (NCBI) 215
RRIDAB_3670769
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP33897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
WB protocol for ABCD1 antibody 83038-4-RRDownload protocol
IF protocol for ABCD1 antibody 83038-4-RRDownload protocol
FC protocol for ABCD1 antibody 83038-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols