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验证数据展示

  • IF Staining of HeLa using CL488-83038-4

    IF Staining of HeLa using CL488-83038-4

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ABCD1 antibody (CL488-83038-4, Clone: 230226E12 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-83038-4

    FC experiment of HeLa using CL488-83038-4

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Abcd1 Recombinant Antibody (CL488-83038-4, Clone:230226E12)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ABCD1 Recombinant antibody

ABCD1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83038-4

产品说明书

CloneNo. 230226E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCD1 Ready-To-Use IHC Kit. ABCD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83038-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83038-4 targets ABCD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 ABCD1 fusion protein Ag0453 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 1
别名 EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1
计算分子量 745 aa, 83 kDa
观测分子量 75 kDa
GenBank蛋白编号BC025358
基因名称 ABCD1
Gene ID (NCBI) 215
RRIDAB_3673174
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP33897
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ABCD1 antibody CL488-83038-4Download protocol
FC protocol for CL Plus 488 ABCD1 antibody CL488-83038-4Download protocol
Standard Protocols
Click here to view our Standard Protocols