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验证数据展示

  • FC experiment of HeLa using 83057-4-RR

    FC experiment of HeLa using 83057-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A549 using 83057-4-RR

    FC experiment of A549 using 83057-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83057-4-RR

    Affinity and Kinetic Characterization of 83057-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83057-4-RR against Human GABRA2 were performed. The affinity constant is below 1 pM.

GABRA2 Recombinant antibody

GABRA2 Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 83057-4-RR

产品说明书

CloneNo. 230158H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

230158H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells, A549 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83057-4-RR targets GABRA2 in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GABRA2 fusion protein Ag29301 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gamma-aminobutyric acid (GABA) A receptor, alpha 2
别名 230158H5
计算分子量 51 kDa
观测分子量50-51 kDa
GenBank蛋白编号NM_000807
基因名称 GABRA2
Gene ID (NCBI) 2555
RRIDAB_3670784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP47869
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

实验方案

Product Specific Protocols
FC protocol for GABRA2 antibody 83057-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols