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GABRA2 Recombinant antibody, PBS Only (Detector)

GABRA2 Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230158H5

Cat no : 83057-4-PBS

Print datasheet

Synonyms

230158H5

验证数据展示

  • FC experiment of HeLa using 83057-4-RR (same clone as 83057-4-PBS)

    FC experiment of HeLa using 83057-4-RR (same clone as 83057-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83057-4-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83057-4-RR (same clone as 83057-4-PBS)

    FC experiment of A549 using 83057-4-RR (same clone as 83057-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human GABRA2 (83057-4-RR, Clone:230158H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83057-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00067-3

    Cytometric bead array standard curve of MP00067-3

    Cytometric bead array standard curve of MP00067-3, GABRA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83057-2-PBS. Detection antibody: 83057-4-PBS. Standard: Ag29301. Range: 0.312-20 ng/mL.

  • Affinity and Kinetic Characterization of 83057-4-PBS

    Affinity and Kinetic Characterization of 83057-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83057-4-PBS against Human GABRA2 was performed. The affinity constant is 0.421 nM.

  • Affinity and Kinetic Characterization of 83057-4-RR

    Affinity and Kinetic Characterization of 83057-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83057-4-RR against Human GABRA2 were performed. The affinity constant is below 1 pM.

产品信息

83057-4-PBS targets GABRA2 as part of a matched antibody pair:

MP00067-3: 83057-2-PBS capture and 83057-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GABRA2 fusion protein Ag29301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 2
Synonyms 230158H5
Calculated Molecular Weight 51 kDa
Observed Molecular Weight50-51 kDa
GenBank Accession NumberNM_000807
Gene Symbol GABRA2
Gene ID (NCBI) 2555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP47869
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.