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GABRA2 Recombinant antibody, PBS Only (Capture/Detector)

GABRA2 Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230158E6

Cat No : 83057-2-PBS

Print datasheet

Synonyms

230158E6

验证数据展示

  • WB analysis using 83057-2-RR (same clone as 83057-2-PBS)

    WB analysis using 83057-2-RR (same clone as 83057-2-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83057-2-PBS in a different storage buffer formulation.

  • WB analysis using 83057-2-RR (same clone as 83057-2-PBS)

    WB analysis using 83057-2-RR (same clone as 83057-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83057-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83057-2-RR (same clone as 83057-2-PBS)

    FC experiment of HeLa using 83057-2-RR (same clone as 83057-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GABRA2 Recombinant antibody (83057-2-RR, Clone:230158E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83057-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00067-2

    Cytometric bead array standard curve of MP00067-2

    Cytometric bead array standard curve ofMP00067-2, GABRA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83057-3-PBS. Detection antibody: 83057-2-PBS. Standard: Ag29301. Range: 0.312-20 ng/mL.

  • Cytometric bead array standard curve of MP00067-3

    Cytometric bead array standard curve of MP00067-3

    Cytometric bead array standard curve ofMP00067-3, GABRA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83057-2-PBS. Detection antibody: 83057-4-PBS. Standard: Ag29301. Range: 0.312-20 ng/mL.

  • Affinity and Kinetic Characterization of 83057-2-RR

    Affinity and Kinetic Characterization of 83057-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83057-2-RR against Human GABRA2 were performed. The affinity constant is 0.403 nM.

产品信息

83057-2-PBS targets GABRA2 as part of a matched antibody pair:

MP00067-2: 83057-3-PBS capture and 83057-2-PBS detection (validated in Cytometric bead array)

MP00067-3: 83057-2-PBS capture and 83057-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Indirect ELISA, WB Application Description
Tested Reactivity rat, mouse, human
Immunogen GABRA2 fusion protein Ag29301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 2
Synonyms 230158E6
Calculated Molecular Weight 51 kDa
GenBank Accession NumberNM_000807
Gene Symbol GABRA2
Gene ID (NCBI) 2555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47869
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C