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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Recombinant antibody, PBS Only (Capture)

Cytokeratin 13 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230366B10

Cat no : 83058-1-PBS

Print datasheet

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

验证数据展示

  • WB analysis using 83058-1-RR (same clone as 83058-1-PBS)

    WB analysis using 83058-1-RR (same clone as 83058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

    IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

    IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • WB analysis of A431 using 83058-1-RR (same clone as 83058-1-PBS)

    WB analysis of A431 using 83058-1-RR (same clone as 83058-1-PBS)

    WB result of Cytokeratin 13 antibody (83058-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00074-2

    Sandwich ELISA standard curve of MP00074-2

    Sandwich ELISA standard curve of MP00074-2, Human Cytokeratin 13 Recombinant Matched Antibody Pair - PBS only. 83058-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0217. 83058-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00074-2

    Sample test of MP00074-2

    The mean Cytokeratin 13 concentration was determined to be 28.00 ng/mL in A431 cell extract based on a 1.50 mg/mL extract load and 12.84 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • IF Staining of HaCaT using 83058-1-RR (same clone as 83058-1-PBS)

    IF Staining of HaCaT using 83058-1-RR (same clone as 83058-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00074-1

    Cytometric bead array standard curve of MP00074-1

    Cytometric bead array standard curve of MP00074-1, Cytokeratin 13 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83058-1-PBS. Detection antibody: 83058-3-PBS. Standard: Ag0217. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83058-1-RR

    Affinity and Kinetic Characterization of 83058-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-1-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.99 nM.

Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

产品信息

83058-1-PBS targets Cytokeratin 13 as part of a matched antibody pair:

MP00074-1: 83058-1-PBS capture and 83058-3-PBS detection (validated in Cytometric bead array)

MP00074-2: 83058-1-PBS capture and 83058-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 13
Synonyms KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13646
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.