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ICAM-1 Recombinant antibody

ICAM-1 Recombinant Antibody for WB,IF/ICC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,IF/ICC,ELISA

Conjugate

Unconjugated

CloneNo.

230342A2

Cat No : 83069-6-RR

Print datasheet

Synonyms

BB2, CD54, ICAM 1, ICAM1, P3.58

验证数据展示

  • WB analysis using 83069-6-RR

    WB analysis using 83069-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83069-6-RR (ICAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83069-6-RR

    IF Staining of HeLa using 83069-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHUVEC cells, Raji cells, Daudi cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83069-6-RR targets ICAM-1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,IF/ICC,ELISA Application Description
Tested Reactivity Human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms BB2, CD54, ICAM 1, ICAM1, P3.58
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
WB protocol for ICAM-1 antibody 83069-6-RRDownload protocol
IF protocol for ICAM-1 antibody 83069-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols