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验证数据展示

  • WB analysis of human placenta using 83096-1-RR

    WB analysis of human placenta using 83096-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83096-1-RR (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83096-1-RR

    FC experiment of HeLa using 83096-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SHBG Recombinant antibody (83096-1-RR, Clone:230356F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83096-1-RR

    Affinity and Kinetic Characterization of 83096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83096-1-RR against Human SHBG were performed. The affinity constant is 0.38 nM.

SHBG Recombinant antibody

SHBG Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83096-1-RR

产品说明书

CloneNo. 230356F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin, SBP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83096-1-RR targets SHBG in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sex hormone-binding globulin
别名 Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin, SBP
计算分子量 402 aa, 44 kDa
观测分子量40-50 kDa
GenBank蛋白编号BC101785
基因名称 SHBG
Gene ID (NCBI) 6462
RRIDAB_3670810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

实验方案

Product Specific Protocols
WB protocol for SHBG antibody 83096-1-RRDownload protocol
FC protocol for SHBG antibody 83096-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols