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CoraLite® Plus 750-conjugated SHBG Recombinant antibody

SHBG Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230356F10

Cat No : CL750-83096

Print datasheet

Synonyms

ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG

验证数据展示

  • WB analysis of human plasma using CL750-83096

    WB analysis of human plasma using CL750-83096

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83096 (SHBG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma
For other applications, we recommend the unconjugated version of this antibody, 83096-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83096 targets SHBG in WB applications and shows reactivity with Human samples.

Tested Applications WB Application Description
Tested Reactivity Human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sex hormone-binding globulin
Synonyms ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG
Calculated Molecular Weight 402 aa, 44 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC101785
Gene Symbol SHBG
Gene ID (NCBI) 6462
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04278
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 SHBG antibody CL750-83096Download protocol
Standard Protocols
Click here to view our Standard Protocols