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验证数据展示

  • WB analysis using 83100-1-RR

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83100-1-RR

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 83100-1-RR

    IF Staining of MCF-7 using 83100-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • FC experiment of A431 using 83100-1-RR

    FC experiment of A431 using 83100-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83100-1-RR

    Affinity and Kinetic Characterization of 83100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

PCYT1A Recombinant antibody

PCYT1A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83100-1-RR

产品说明书

CloneNo. 230393B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

PCYT1, CTPCT, CT A, CCT alpha, CCT A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, mouse testis tissue, mouse brain tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83100-1-RR targets PCYT1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 PCYT1A fusion protein Ag34268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphate cytidylyltransferase 1, choline, alpha
别名 PCYT1, CTPCT, CT A, CCT alpha, CCT A
计算分子量42 kDa
观测分子量38-41 kDa
GenBank蛋白编号NM_005017
基因名称 PCYT1A
Gene ID (NCBI) 5130
RRIDAB_3670813
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49585
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCYT1A antibody 83100-1-RRDownload protocol
IF protocol for PCYT1A antibody 83100-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols