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PCYT1A Recombinant antibody

PCYT1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230393B4

Cat no : 83100-1-RR

Print datasheet

Synonyms

PCYT1, CTPCT, CT A, CCT alpha, CCT A

验证数据展示

  • WB analysis using 83100-1-RR

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83100-1-RR

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 83100-1-RR

    IF Staining of MCF-7 using 83100-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 83100-1-RR

    FC experiment of A431 using 83100-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83100-1-RR

    Affinity and Kinetic Characterization of 83100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, mouse testis tissue, mouse brain tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83100-1-RR targets PCYT1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCYT1A fusion protein Ag34268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphate cytidylyltransferase 1, choline, alpha
Synonyms PCYT1, CTPCT, CT A, CCT alpha, CCT A
Calculated Molecular Weight42 kDa
Observed Molecular Weight38-41 kDa
GenBank Accession NumberNM_005017
Gene Symbol PCYT1A
Gene ID (NCBI) 5130
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCYT1A antibody 83100-1-RRDownload protocol
IF protocol for PCYT1A antibody 83100-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols