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验证数据展示

  • IF Staining of MCF-7 using CL488-83100

    IF Staining of MCF-7 using CL488-83100

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PCYT1A antibody (CL488-83100, Clone: 230393B4 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-83100

    FC experiment of A431 using CL488-83100

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pcyt1a Recombinant Antibody (CL488-83100, Clone:230393B4)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated PCYT1A Recombinant antibody

PCYT1A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83100

产品说明书

CloneNo. 230393B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

PCYT1, CTPCT, CT A, CCT alpha, CCT A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 83100-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83100 targets PCYT1A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 PCYT1A fusion protein Ag34268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphate cytidylyltransferase 1, choline, alpha
别名 PCYT1, CTPCT, CT A, CCT alpha, CCT A
计算分子量42 kDa
观测分子量38-41 kDa
GenBank蛋白编号NM_005017
基因名称 PCYT1A
Gene ID (NCBI) 5130
RRIDAB_3673190
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49585
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PCYT1A antibody CL488-83100Download protocol
FC protocol for CL Plus 488 PCYT1A antibody CL488-83100Download protocol
Standard Protocols
Click here to view our Standard Protocols