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NEDD4 Recombinant antibody, PBS Only (Detector)

NEDD4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230478D8

Cat no : 83112-4-PBS

Print datasheet

Synonyms

KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein

验证数据展示

  • WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-4-RR (NEDD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    WB analysis using 83112-4-RR (same clone as 83112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-4-RR (NEDD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    FC experiment of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug NEDD4 Recombinant antibody (83112-4-RR, Clone:230478D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-4-RR, Clone: 230478D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    IF Staining of U2OS using 83112-4-RR (same clone as 83112-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-4-RR, Clone: 230478D8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83112-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1

    Cytometric bead array standard curve of MP00234-1, NEDD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83112-2-PBS. Detection antibody: 83112-4-PBS. Standard: Ag16291. Range: 1.25-20 ng/mL.

产品信息

83112-4-PBS targets NEDD4 as part of a matched antibody pair:

MP00234-1: 83112-2-PBS capture and 83112-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NEDD4 fusion protein Ag16291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 4
Synonyms KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein
Calculated Molecular Weight 1319 aa, 149 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC152452
Gene Symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46934
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).