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OGT Recombinant antibody

OGT Recombinant Antibody for WB, IF/ICC, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230385D11

Cat No : 83113-1-RR

Print datasheet

Synonyms

FLJ23071, HRNT1, OGT

验证数据展示

  • WB analysis using 83113-1-RR

    WB analysis using 83113-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83113-1-RR (OGT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83113-1-RR

    IF Staining of HeLa using 83113-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OGT antibody (83113-1-RR, Clone: 230385D11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83113-1-RR

    FC experiment of HeLa using 83113-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug OGT Recombinant antibody (83113-1-RR, Clone:230385D11)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells, Jurkat cells, U-937 cells, mouse brain tissue, rat brain tissue
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83113-1-RR targets OGT in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen OGT fusion protein Ag2160 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms FLJ23071, HRNT1, OGT
Calculated Molecular Weight 1046 aa, 117 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC014434
Gene Symbol OGT
Gene ID (NCBI) 8473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

实验方案

Product Specific Protocols
WB protocol for OGT antibody 83113-1-RRDownload protocol
IF protocol for OGT antibody 83113-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols