• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83113-1-RR (same clone as 83113-1-PBS)

    WB analysis using 83113-1-RR (same clone as 83113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83113-1-RR (OGT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

    IF Staining of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OGT antibody (83113-1-RR, Clone: 230385D11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

    FC experiment of HeLa using 83113-1-RR (same clone as 83113-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug OGT Recombinant antibody (83113-1-RR, Clone:230385D11)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83113-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83113-1-RR

    Affinity and Kinetic Characterization of 83113-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83113-1-RR against Human OGT were performed. The affinity constant is below 1 pM.

OGT Recombinant antibody, PBS Only

OGT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83113-1-PBS

产品说明书

CloneNo. 230385D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.

产品信息

83113-1-PBS targets OGT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OGT fusion protein Ag2160 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
别名 O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255
计算分子量 1046 aa, 117 kDa
观测分子量 110 kDa
GenBank蛋白编号BC014434
基因名称 OGT
Gene ID (NCBI) 8473
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15294
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.