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TLR3 Recombinant antibody

TLR3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230445G4

Cat No : 83136-3-RR

Print datasheet

Synonyms

CD283, TLR3, toll like receptor 3

验证数据展示

  • WB analysis using 83136-3-RR

    WB analysis using 83136-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83136-3-RR

    Affinity experiments of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83136-3-RR targets TLR3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TLR3 fusion protein Ag34044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll-like receptor 3
Synonyms CD283, TLR3, toll like receptor 3
Calculated Molecular Weight 904 aa, 104 kDa
Observed Molecular Weight120 kDa, 68 kDa
GenBank Accession NumberBC094737
Gene Symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15455
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).

实验方案

Product Specific Protocols
WB protocol for TLR3 antibody 83136-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols