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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83136-3-RR

    Affinity and Kinetic Characterization of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

TLR3 Recombinant antibody, PBS Only

TLR3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83136-3-PBS

产品说明书

CloneNo. 230445G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

230445G4, CD283, toll like receptor 3, Toll-like receptor 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83136-3-PBS targets TLR3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 TLR3 fusion protein Ag34044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor 3
别名 230445G4, CD283, toll like receptor 3, Toll-like receptor 3
计算分子量 904 aa, 104 kDa
观测分子量120 kDa, 68 kDa
GenBank蛋白编号BC094737
基因名称 TLR3
Gene ID (NCBI) 7098
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15455
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).