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TLR3 Recombinant antibody, PBS Only

TLR3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230445G4

Cat no : 83136-3-PBS

Print datasheet

Synonyms

Toll-like receptor 3, toll like receptor 3, CD283, 230445G4

验证数据展示

  • WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    WB analysis using 83136-3-RR (same clone as 83136-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    IHC staining of human ovarian cancer using 83136-3-RR (same clone as 83136-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83136-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83136-3-RR

    Affinity and Kinetic Characterization of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

产品信息

83136-3-PBS targets TLR3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TLR3 fusion protein Ag34044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll-like receptor 3
Synonyms Toll-like receptor 3, toll like receptor 3, CD283, 230445G4
Calculated Molecular Weight 904 aa, 104 kDa
Observed Molecular Weight120 kDa, 68 kDa
GenBank Accession NumberBC094737
Gene Symbol TLR3
Gene ID (NCBI) 7098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15455
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).