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验证数据展示

  • WB analysis using 83136-3-RR

    WB analysis using 83136-3-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83136-3-RR

    IHC staining of human ovarian cancer using 83136-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovarian cancer using 83136-3-RR

    IHC staining of human ovarian cancer using 83136-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83136-3-RR

    Affinity and Kinetic Characterization of 83136-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

TLR3 Recombinant antibody

TLR3 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83136-3-RR

Print datasheet

CloneNo. 230445G4
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Toll-like receptor 3, toll like receptor 3, CD283, 230445G4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, human placenta tissue
Positive IHC detected inhuman ovarian cancer, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83136-3-RR targets TLR3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TLR3 fusion protein Ag34044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll-like receptor 3
Synonyms Toll-like receptor 3, toll like receptor 3, CD283, 230445G4
Calculated Molecular Weight 904 aa, 104 kDa
Observed Molecular Weight120 kDa, 68 kDa
GenBank Accession NumberBC094737
Gene Symbol TLR3
Gene ID (NCBI) 7098
RRIDAB_3670837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15455
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).

实验方案

Product Specific Protocols
WB protocol for TLR3 antibody 83136-3-RRDownload protocol
IHC protocol for TLR3 antibody 83136-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

NAR Mol Med

C G composition in transposon-derived genes is increased in FXD with perturbed immune system

Authors - Tamaki Suganuma
View Article