TLR3 Recombinant antibody

TLR3 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83136-3-RR

产品说明书

CloneNo. 230445G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

230445G4, CD283, toll like receptor 3, Toll-like receptor 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, human placenta tissue
Positive IHC detected inhuman ovarian cancer, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

83136-3-RR targets TLR3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 TLR3 fusion protein Ag34044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll-like receptor 3
别名 230445G4, CD283, toll like receptor 3, Toll-like receptor 3
计算分子量 904 aa, 104 kDa
观测分子量120 kDa, 68 kDa
GenBank蛋白编号BC094737
基因名称 TLR3
Gene ID (NCBI) 7098
RRIDAB_3670837
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15455
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).

实验方案

Product Specific Protocols
WB protocol for TLR3 antibody 83136-3-RRDownload protocol
IHC protocol for TLR3 antibody 83136-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

NAR Mol Med

C G composition in transposon-derived genes is increased in FXD with perturbed immune system

Authors - Tamaki Suganuma
Loading...