验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, human placenta tissue |
Positive IHC detected in | human ovarian cancer, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:14000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
83136-3-RR targets TLR3 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | TLR3 fusion protein Ag34044 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | toll-like receptor 3 |
别名 | 230445G4, CD283, toll like receptor 3, Toll-like receptor 3 |
计算分子量 | 904 aa, 104 kDa |
观测分子量 | 120 kDa, 68 kDa |
GenBank蛋白编号 | BC094737 |
基因名称 | TLR3 |
Gene ID (NCBI) | 7098 |
RRID | AB_3670837 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | O15455 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).
实验方案
Product Specific Protocols | |
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WB protocol for TLR3 antibody 83136-3-RR | Download protocol |
IHC protocol for TLR3 antibody 83136-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |