TLR3 Recombinant antibody
TLR3 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
230445G4
验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, human placenta tissue |
| Positive IHC detected in | human ovarian cancer, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83136-3-RR targets TLR3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | TLR3 fusion protein Ag34044 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | toll-like receptor 3 |
| Synonyms | Toll-like receptor 3, toll like receptor 3, CD283, 230445G4 |
| Calculated Molecular Weight | 904 aa, 104 kDa |
| Observed Molecular Weight | 120 kDa, 68 kDa |
| GenBank Accession Number | BC094737 |
| Gene Symbol | TLR3 |
| Gene ID (NCBI) | 7098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O15455 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TLR3 antibody 83136-3-RR | Download protocol |
| IHC protocol for TLR3 antibody 83136-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
