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验证数据展示

  • WB analysis of mouse pancreas using 83182-5-RR

    WB analysis of mouse pancreas using 83182-5-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83182-5-RR (SLC38A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83182-5-RR

    FC experiment of U2OS using 83182-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Slc38a9 Recombinant Antibody (83182-5-RR, Clone:240039E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83182-5-RR

    Affinity and Kinetic Characterization of 83182-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83182-5-RR against Human SLC38A9 were performed. The affinity constant is 0.715 nM.

SLC38A9 Recombinant monoclonal antibody

SLC38A9 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83182-5-RR

产品说明书

CloneNo. 240039E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83182-5-RR targets SLC38A9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15607

Product name: Recombinant human SLC38A9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-131 aa of BC066891

Sequence: MANMNSDSRHLGTSEVDHERDPGPMNIQFEPSDLRSKRPFCIEPTNIVNVNHVIQRVSDHASAMNKRIHYYSRLTTPADKALIPPDHVVPAPEECYVYSPLGSAYKLQSYTEGYGKNTSLVTIFMIWNTMM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 38, member 9
别名 URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7
计算分子量 561 aa, 64 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC066891
基因名称 SLC38A9
Gene ID (NCBI) 153129
RRIDAB_3670872
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NBW4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that responds to multiple environmental cues. The lysosomal amino acid transporter SLC38A9 is a physical and functional component of this sensing complex. SLC38A9 (Solute Carrier Family 38 Member 9) was first identified as a potential amino acid transporter belonging to the slc38 family. SLC38A9 has some isoforms: 64,53,57 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC38A9 antibody 83182-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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