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验证数据展示

  • WB analysis using 83189-3-RR

    WB analysis using 83189-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83189-3-RR (SORBS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83189-3-RR

    IF Staining of U2OS using 83189-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SORBS2 antibody (83189-3-RR, Clone: 240023A8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U2OS using 83189-3-RR

    FC experiment of U2OS using 83189-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SORBS2 Recombinant antibody (83189-3-RR, Clone:240023A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83189-3-RR

    Affinity and Kinetic Characterization of 83189-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83189-3-RR against Human SORBS2 were performed. The affinity constant is 0.433 nM.

SORBS2 Recombinant antibody

SORBS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83189-3-RR

产品说明书

CloneNo. 240023A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, HuH-7 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SORBS2 Ready-To-Use IHC Kit. SORBS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83189-3-RR targets SORBS2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SORBS2 fusion protein Ag20041 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sorbin and SH3 domain containing 2
别名 Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8
计算分子量 1100 aa, 124 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC011883
基因名称 SORBS2
Gene ID (NCBI) 8470
RRIDAB_3670879
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO94875
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

实验方案

Product Specific Protocols
WB protocol for SORBS2 antibody 83189-3-RRDownload protocol
IF protocol for SORBS2 antibody 83189-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols