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CoraLite® Plus 488-conjugated SORBS2 Recombinant antibody

SORBS2 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240023A8

Cat No : CL488-83189-3

Print datasheet

Synonyms

Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8

验证数据展示

  • IF Staining of U2OS using CL488-83189-3

    IF Staining of U2OS using CL488-83189-3

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SORBS2 antibody (CL488-83189-3, Clone: 240023A8 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SORBS2 Ready-To-Use IHC Kit. SORBS2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83189-3-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83189-3 is SORBS2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen SORBS2 fusion protein Ag20041 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sorbin and SH3 domain containing 2
Synonyms Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8
Calculated Molecular Weight 1100 aa, 124 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC011883
Gene Symbol SORBS2
Gene ID (NCBI) 8470
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94875
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SORBS2 antibody CL488-83189-3Download protocol
Standard Protocols
Click here to view our Standard Protocols