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NUDCD2 Recombinant antibody

NUDCD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230513A5

Cat no : 83217-5-RR

Print datasheet

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5

验证数据展示

  • FC experiment of HepG2 using 83217-5-RR

    FC experiment of HepG2 using 83217-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 83217-5-RR

    WB analysis using 83217-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 83217-5-RR

    IP experiment of HepG2 using 83217-5-RR

    IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug.

  • IF Staining of HepG2 using 83217-5-RR

    IF Staining of HepG2 using 83217-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83217-5-RR

    Affinity and Kinetic Characterization of 83217-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, U-251 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83217-5-RR is NUDCD2 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen NUDCD2 fusion protein Ag15579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NudC domain containing 2
Synonyms NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5
Calculated Molecular Weight 157 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC017934
Gene Symbol NUDCD2
Gene ID (NCBI) 134492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8WVJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

实验方案

Product Specific Protocols
WB protocol for NUDCD2 antibody 83217-5-RRDownload protocol
IF protocol for NUDCD2 antibody 83217-5-RRDownload protocol
IP protocol for NUDCD2 antibody 83217-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols