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NUDCD2 Recombinant antibody, PBS Only

NUDCD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230513A5

Cat no : 83217-5-PBS

Print datasheet

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5

验证数据展示

  • FC experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    FC experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • WB analysis using 83217-5-RR (same clone as 83217-5-PBS)

    WB analysis using 83217-5-RR (same clone as 83217-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    IP experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    IF Staining of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83217-5-RR

    Affinity and Kinetic Characterization of 83217-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

产品信息

The immunogen of 83217-5-PBS is NUDCD2 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human
Immunogen NUDCD2 fusion protein Ag15579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NudC domain containing 2
Synonyms NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5
Calculated Molecular Weight 157 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC017934
Gene Symbol NUDCD2
Gene ID (NCBI) 134492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8WVJ2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).