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RILPL1 Recombinant antibody

RILPL1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230515D1

Cat No : 83220-6-RR

Print datasheet

Synonyms

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

验证数据展示

  • WB analysis using 83220-6-RR

    WB analysis using 83220-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83220-6-RR (RILPL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83220-6-RR

    WB analysis of HepG2 using 83220-6-RR

    WB result of RILPL1 antibody (83220-6-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 83220-6-RR

    Affinity and Kinetic Characterization of 83220-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83220-6-RR against Human RILPL1 were performed. The affinity constant is 1.97 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, A549 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83220-6-RR is RILPL1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RILPL1 fusion protein Ag10192 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Rab interacting lysosomal protein-like 1
Synonyms RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378
Calculated Molecular Weight 252 aa, 29 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC080626
Gene Symbol RILPL1
Gene ID (NCBI) 353116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5EBL4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein localization in the primary cilium. Rilpl2 was initially identified as up-regulated in ciliating mouse tracheal epithelial cells. Rilpl1 and Rilpl2 both localize to the primary cilium and centrosome, Rilpl1 specifically to the distal end of the mother centriole.(PMID:23264467)

实验方案

Product Specific Protocols
WB protocol for RILPL1 antibody 83220-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols