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验证数据展示

  • FC experiment of HeLa using 83241-1-RR

    FC experiment of HeLa using 83241-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SH-SY5Y using 83241-1-RR

    FC experiment of SH-SY5Y using 83241-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83241-1-RR

    Affinity and Kinetic Characterization of 83241-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

Glypican 2 Recombinant antibody

Glypican 2 Uni-rAbTM Recombinant Antibody for ELISA
Cat No. 83241-1-RR

产品说明书

CloneNo. 230518A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

ELISA

别名

GPC2, Secreted glypican-2, FLJ38962, 230518A4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells, HeLa cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83241-1-RR targets Glypican 2 in ELISA applications and shows reactivity with human samples.

经测试应用 ELISA Application Description
经测试反应性 human
免疫原 Glypican 2 fusion protein Ag4041 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=1.07 x 105M
全称 glypican 2
别名 GPC2, Secreted glypican-2, FLJ38962, 230518A4
计算分子量 579 aa, 63 kDa
观测分子量62，70-100 kDa
GenBank蛋白编号BC027972
基因名称 GPC2
Gene ID (NCBI) 221914
RRIDAB_3670917
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N158
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).

实验方案

Product Specific Protocols
FC protocol for Glypican 2 antibody 83241-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols