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IL3RA/CD123 Recombinant antibody

IL3RA/CD123 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240042E5

Cat no : 83243-3-RR

Print datasheet

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

验证数据展示

  • WB analysis of HL-60 using 83243-3-RR

    WB analysis of HL-60 using 83243-3-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83243-3-RR

    Affinity and Kinetic Characterization of 83243-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

经过测试的应用

Positive WB detected inHL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83243-3-RR is IL3RA/CD123 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen IL3RA/CD123 fusion protein Ag4452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 receptor, alpha (low affinity)
Synonyms IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra
Calculated Molecular Weight 378 aa, 43 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC035407
Gene Symbol CD123
Gene ID (NCBI) 3563
ENSEMBL Gene IDENSG00000185291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26951
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

实验方案

Product Specific Protocols
WB protocol for IL3RA/CD123 antibody 83243-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols