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IL3RA/CD123 Recombinant antibody, PBS Only (Capture)

IL3RA/CD123 Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240042E5

Cat no : 83243-3-PBS

Print datasheet

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

验证数据展示

  • WB analysis of HL-60 using 83243-3-RR (same clone as 83243-3-PBS)

    WB analysis of HL-60 using 83243-3-RR (same clone as 83243-3-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00176-4

    Sandwich ELISA standard curve of MP00176-4

    Sandwich ELISA standard curve of MP00176-4, Human IL3RA Recombinant Matched Antibody Pair - PBS only. 83243-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4452. 83243-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP00176-4

    Sample test of MP00176-4

    THP-1 and HL-60 cell lysates were measured. The human ILR3A concentration of detected samples was determined to be 76,268.8 pg/mL (based on a 4.0 mg/mL extract load) in THP-1 cell lysate and 39,279.2 pg/mL (based on a 4.3 mg/mL extract load) in HL-60 cell lysate.

  • IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83243-3-RR

    Affinity and Kinetic Characterization of 83243-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

产品信息

83243-3-PBS targets IL3RA/CD123 as part of a matched antibody pair:

MP00176-4: 83243-3-PBS capture and 83243-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen IL3RA/CD123 fusion protein Ag4452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 receptor, alpha (low affinity)
Synonyms IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra
Calculated Molecular Weight 378 aa, 43 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC035407
Gene Symbol CD123
Gene ID (NCBI) 3563
ENSEMBL Gene IDENSG00000185291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26951
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.