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验证数据展示

  • WB analysis of HL-60 using 83243-3-RR (same clone as 83243-3-PBS)

    WB analysis of HL-60 using 83243-3-RR (same clone as 83243-3-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00176-4

    Sandwich ELISA standard curve of MP00176-4

    Sandwich ELISA standard curve of MP00176-4, Human IL3RA Recombinant Matched Antibody Pair - PBS only. 83243-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4452. 83243-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP00176-4

    Sample test of MP00176-4

    THP-1 and HL-60 cell lysates were measured. The human ILR3A concentration of detected samples was determined to be 76,268.8 pg/mL (based on a 4.0 mg/mL extract load) in THP-1 cell lysate and 39,279.2 pg/mL (based on a 4.3 mg/mL extract load) in HL-60 cell lysate.

  • IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    IHC staining of human tonsillitis using 83243-3-RR (same clone as 83243-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83243-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83243-3-RR

    Affinity and Kinetic Characterization of 83243-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

IL3RA/CD123 Recombinant antibody, PBS Only (Capture)

IL3RA/CD123 Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83243-3-PBS

产品说明书

CloneNo. 240042E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

别名

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83243-3-PBS targets IL3RA/CD123 as part of a matched antibody pair:

MP00176-4: 83243-3-PBS capture and 83243-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 IL3RA/CD123 fusion protein Ag4452 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 3 receptor, alpha (low affinity)
别名 IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra
计算分子量 378 aa, 43 kDa
观测分子量70 kDa
GenBank蛋白编号BC035407
基因名称 CD123
Gene ID (NCBI) 3563
ENSEMBL Gene IDENSG00000185291
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP26951
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.