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RPN1 Recombinant antibody

RPN1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240044D4

Cat No : 83250-2-RR

Print datasheet

Synonyms

DKFZp686B16177, hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1

验证数据展示

  • WB analysis using 83250-2-RR

    WB analysis using 83250-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83250-2-RR

    Affinity experiments of 83250-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83250-2-RR targets RPN1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RPN1 fusion protein Ag3576 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribophorin I
Synonyms DKFZp686B16177, hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 66-69 kDa
GenBank Accession NumberBC010839
Gene Symbol RPN1
Gene ID (NCBI) 6184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04843
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPN1 antibody 83250-2-RRDownload protocol
FC protocol for RPN1 antibody 83250-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols