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验证数据展示

  • FC experiment of HeLa using 83250-2-RR

    FC experiment of HeLa using 83250-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 83250-2-RR

    WB analysis using 83250-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83250-2-RR

    IHC staining of human stomach cancer using 83250-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83250-2-RR

    Affinity and Kinetic Characterization of 83250-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

Ribophorin I Recombinant antibody

Ribophorin I Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83250-2-RR

产品说明书

CloneNo. 240044D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83250-2-RR targets Ribophorin I in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Ribophorin I fusion protein Ag3576 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribophorin I
别名 RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
计算分子量 69 kDa
观测分子量 66-69 kDa
GenBank蛋白编号BC010839
基因名称 RPN1
Gene ID (NCBI) 6184
RRIDAB_3670925
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04843
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Ribophorin I antibody 83250-2-RRDownload protocol
IHC protocol for Ribophorin I antibody 83250-2-RRDownload protocol
FC protocol for Ribophorin I antibody 83250-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols