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Ribophorin I Recombinant antibody, PBS Only (Capture/Detector)

Ribophorin I Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240044D4

Cat no : 83250-2-PBS

Print datasheet

Synonyms

RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit

验证数据展示

  • FC experiment of HeLa using 83250-2-RR (same clone as 83250-2-PBS)

    FC experiment of HeLa using 83250-2-RR (same clone as 83250-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

  • WB analysis using 83250-2-RR (same clone as 83250-2-PBS)

    WB analysis using 83250-2-RR (same clone as 83250-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00231-3

    Sandwich ELISA standard curve of MP00231-3

    Sandwich ELISA standard curve of MP00231-3, Human Ribophorin I Recombinant Matched Antibody Pair - PBS only. 83250-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3576. 83250-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00231-3

    Sample test of MP00231-3

    The mean Ribophorin I concentration was determined to be 145.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 11.0 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

  • IHC staining of human stomach cancer using 83250-2-RR (same clone as 83250-2-PBS)

    IHC staining of human stomach cancer using 83250-2-RR (same clone as 83250-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00231-2

    Cytometric bead array standard curve of MP00231-2

    Cytometric bead array standard curve of MP00231-2, RPN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83250-2-PBS. Detection antibody: 83250-1-PBS. Standard: Ag3576. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83250-2-PBS

    Affinity and Kinetic Characterization of 83250-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83250-2-PBS against Human Ribophorin I was performed. The affinity constant is 1.69 nM.

  • Affinity and Kinetic Characterization of 83250-2-RR

    Affinity and Kinetic Characterization of 83250-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

产品信息

83250-2-PBS targets Ribophorin I as part of a matched antibody pair:

MP00231-2: 83250-2-PBS capture and 83250-1-PBS detection (validated in Cytometric bead array)

MP00231-3: 83250-1-PBS capture and 83250-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen Ribophorin I fusion protein Ag3576 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribophorin I
Synonyms RPN1, RPN-I, Ribophorin-1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 66-69 kDa
GenBank Accession NumberBC010839
Gene Symbol RPN1
Gene ID (NCBI) 6184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04843
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C