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验证数据展示

  • WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    IF Staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD19 antibody (83254-1-RR, Clone: 240135A3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00253-1

    Cytometric bead array standard curve of MP00253-1

    Cytometric bead array standard curve of MP00253-1, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-2-PBS. Detection antibody: 83254-1-PBS. Standard: Eg0907. Range: 2.5-80 ng/mL.

  • Cytometric bead array standard curve of MP00253-3

    Cytometric bead array standard curve of MP00253-3

    Cytometric bead array standard curve of MP00253-3, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-1-PBS. Detection antibody: 83254-4-PBS. Standard: Eg0907. Range: 5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83254-1-RR

    Affinity and Kinetic Characterization of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

Mouse CD19 Recombinant antibody, PBS Only (Capture/Detector)

CD19 Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 83254-1-PBS

产品说明书

CloneNo. 240135A3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

CD19 antigen, 240135A3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83254-1-PBS targets CD19 as part of a matched antibody pair:

MP00253-1: 83254-2-PBS capture and 83254-1-PBS detection (validated in Cytometric bead array)

MP00253-3: 83254-1-PBS capture and 83254-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD19 antigen
别名 CD19 antigen, 240135A3
计算分子量60 kDa
观测分子量62-70 kDa
GenBank蛋白编号NM_009844.2
基因名称 Cd19
Gene ID (NCBI) 12478
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25918
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).