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验证数据展示

  • WB analysis using 83254-1-RR

    WB analysis using 83254-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 83254-1-RR

    IHC staining of rat spleen using 83254-1-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83254-1-RR

    IHC staining of mouse spleen using 83254-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83254-1-RR

    IHC staining of mouse spleen using 83254-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 83254-1-RR

    IF Staining of mouse spleen using 83254-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD19 antibody (83254-1-RR, Clone: 240135A3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83254-1-RR

    Affinity and Kinetic Characterization of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

CD19 Recombinant antibody

CD19 Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83254-1-RR

产品说明书

CloneNo. 240135A3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CD19 antigen, 240135A3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83254-1-RR targets CD19 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD19 antigen
别名 CD19 antigen, 240135A3
计算分子量60 kDa
观测分子量62-70 kDa
GenBank蛋白编号NM_009844.2
基因名称 Cd19
Gene ID (NCBI) 12478
RRIDAB_3670927
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25918
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

实验方案

Product Specific Protocols
WB protocol for CD19 antibody 83254-1-RRDownload protocol
IHC protocol for CD19 antibody 83254-1-RRDownload protocol
IF protocol for CD19 antibody 83254-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols